An entrepreneurial mindset is particularly important in modern occupations, whether to expand an existing business or to embark on a new venture. This paper proposes a neural marker reflecting individuals’ entrepreneurial mindset (EM) fit by integrating psychometric and neurocognitive approaches. Building on research linking EM traits—such as opportunity recognition, resilience, and risk-taking—with brain activity, the suggested framework utilizes EEG-based analyses to investigate functional and effective connectivity across twelve established neural networks. Key metrics, including coherence, phase relationships, and phase slope index, are employed to develop discriminant functions that distinguish high- and low-EM individuals. The methodology introduces the Entrepreneurial Mindset Index (EMI), a composite measure of Z-scores reflecting optimal neural connectivity profiles. The approach offers a foundation for future research and practical applications, including EEG biofeedback interventions to enhance entrepreneurial capacities through targeted neuroplasticity.