This study explores how different goal orientations—Learning Goal Orientation (LGO), Performance-Prove Goal Orientation (PGO), and Performance-Avoid Goal Orientation (PAGO)—relate to self-efficacy, optimism, and well-being outcomes, specifically burnout and flourishing. Using a sample of 332 participants, including college students and working adults, we examined a serial mediation model to investigate the roles of self-efficacy and optimism as mediators. The findings revealed that LGO positively influences flourishing by enhancing self-efficacy and optimism. In contrast, PAGO suggested increased burnout with minimal direct mediation effects. Additionally, the results from PGO did not show a significant impact on either burnout or flourishing. Together, these results provide valuable insights into how motivational orientations and psychological resources contribute to workplace well-being. This study provides practical implications for organizations that can foster LGO through enhanced interventions and mitigate the detrimental effects of PAGO by strengthening self-efficacy and optimism.